Detection Kit for Fibronectin(FN)
【Product Name】Detection Kit for FN (Chemiluminescence immunoassay)
【registration certificate number】 京械注准20172401058
【Packing】48 tests/box,96 tests/box.
【Storage conditions and validity】Storage at 2 ~ 8℃,validity for 6 months.
This kit is mainly used for quantitative determination of fibronectin (FN) in human serum in vitro.
Fibronectin (FN), a cold-insoluble globulin, is a family of high molecular weight glycoproteins secreted by mesenchymal cells and widely distributed on the cell surface (cytotype FN · CFn) and plasma (plasma FN · PFn ).
Fibronectin has function of cell adhesion and proliferation, plasma FN mainly comes out of vascular endothelial cells and macrophages, it promote not only the connection of cell with fiber or matrix, but also the phagocytosis of macrophages and it is closely related to wound healing, tissue inflammation, the process of fibrosis and hardening.
The kit uses sandwich method, the reagents used in the kit is the FN standard, the microplate is coated by FN, horseradish peroxidase markers and chemiluminescent substrate (with enhancer). FN (standard or test sample) and enzyme conjugate was added to microplate, solid-phase antibody in the microplate, antigen in the sample to be tested (or standard) and the enzyme marker will form a complex (antibody-antigen-HRP-FN). The more antigens in the sample to be tested, the more antibody-antigen-HRP-FN are formed and vice versa. After immune reaction, unbound labeled-FN were removed and the optical signal can be produced once adding chemiluminescent substrate, the optical signal will increase with the antigen content of the sample to be tested. According to the relative luminescence intensity and its concentration of FN series standards, the dose-response curve can be drawn by log-logit mathematical model or other suitable mathematical model fitting. The content of FN in the sample to be tested can be calculated from this curve by its relative luminescence intensity.
1.Repair and healing of wounds: a large number of studies and clinical applications have shown that FN plays an important role in the repair and healing of wounds and is the key to promoting wound healing.
2.Diagnosis and treatment of cancer: a large number of studies have shown that the distribution and content of FN in vivo are closely related to the occurrence and development of tumors.
3. The treatment of blood system and cardiovascular diseases: experiments show that plasma FN can be added to the blood of patients with EDS to achieve the coagulation of platelets and alleviate the disease.
4. For adjuvant treatment of sepsis, shock and other first aid: when the body appear large trauma, infection and associated with sepsis, shock, levels of FN in patients' blood fell sharply and make the body extremely weak, then it can't resist the infection.
5. FN and cervical erosion, cervical erosion is a common gynecological diseases, past statistics showed that disease groups were among women aged 35 ~ 50, in recent years, according to clinical statistic, gynecological diseases among 17 to 23 years old , the incidence of cervical erosion is as high as 90%, it has been a serious threat to women's health and life.
6. The relationship between FN and diabetes and other diseases, according to research at home and abroad, the synthesis of diabetic fibroblast extracellular matrix protein changes, the proportion of FN and collagen is reduced, as well as an increase of collagen relative content.
Clinical laboratory, physical examination center